Description

A superior DH5a strain with a transformation efficiency similar to that of electroporation(~10¹⁰ cfu/ug)!

ECOS™ X can be utilized for applications that require very high transformation efficiencies, such as genomic library construction. The “X” as shown in the name, stands for extreme, meaning this competent cell was designed to exceed and set new limitations! The “X” also represents “10” in Roman numeral; our new product aims to reach 10¹⁰ in its efficiency.

Genotype

endA1 recA1 relA1 deoR gyrA96 nupG hsdR17(rk^–, mk⁺) supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 lacZΔM15 λ^– F^–

Features

• Super high efficiency comparable to electro-competent cells
• One step, one tube, finish in 6 minutes
• Requires no costly electroporation equipment

Applications

• Efficient transformation of products from routine TA cloning
• Transformation of minute amounts of PCR products
• Construction of a more representative gene library

Specifications

Species    E. coli

Strain       DH5α

Efficiency   >5 x 10⁹ cfu/μg (FYE610-10VL / -80VL)

Format     Tube (100 μL) 

Quantity   10 x 100 μL  / 80 x 100 μL

Store at    -70°C.