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ECOS™ X Competent Cells [DH5α]
A superior DH5a strain with a transformation efficiency similar to that of electroporation(~10^10 cfu/ug) ！
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Description
ECOS™ X can be utilized for applications which require very high transformation efficiencies, such as of genomic library construction . The “X” as shown in the name, stands for extreme, meaning this competent cell was designed to exceed and set new limitations! The “X” also represents “10” in Roman numeral; our new product aims to reach 1010 in its efficiency.
Genotype
endA1 recA1 relA1 deoR gyrA96 nupG hsdR17(rk–, mk+) supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 lacZΔM15 λ– F–
Features
- Super high efficiency comparable to electro-competent cells
- One step, one tube, finish in 6 minutes
- Requires no costly electroporation equipment
Applications
- Efficient transformation of products from routine TA cloning
- Transformation of minute amounts of PCR products
- Construction of a more representative gene library
Specifications
Species E. coli
Strain DH5α
Efficiency ~ 5 x 109 cfu/μg (FYE610-10VL / FYE610-80VL)
Format Tube (100 μL)
Quantity 10 x 100 μL、80 x 100 μL
Store at -70°C.
Content
- 100 µL / vial ECOSTM Competent Cells
- 5 µL pUC19 Control plasmid (10-4 μg / µL)
- ECOSTM patented protocol
