ECOS™ St Competent Cells [HB101 Derived]
Description
ECOS™ St Competent Cells is a strain derived from HB101 and suitable for cloning unstable inserts such as lentiviral DNA with LTR (Long terminal repeat).
ECOS™ Competent Cells are chemically competent cells, which can be used with quick transformation protocol and no need recovery step. There are two transformation efficiencies of this strain in the ECOS™ product line. The item FYE307 is suitable for general use. If you want to transform longer plasmids, plasmids with a kanamycin selection system, or get maximum quantity colonies, you have an alternative option, HYE007-S08, the efficiency is 1*109 cfu/μg. This product can perfectly replace Stbl3™ of Thermo Fisher or Endura™ of Lucigen.
Genotype
F– mcrB mrr hsdS20(rB– ,mB– ) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR ) xyl-5 λ– leu mtl-1
Features and Advantages
- Suitable for lentivirus or retrovirus cloning
- The highest efficiency in the same strains of chemically competent cells in the world
- 6-minute operation protocol can achieve the highest efficiency
Applications
- Lentivirus and retrovirus cloning
Efficiency
>1 x 108 cfu/ μg Recommended for:
- Subcloning
- General cloning
>1 x 109 cfu/ μg Recommended for:
- Super high-efficiency cloning
- Cloning lowest abundance transcripts
- Gene library
- Kanamycin-selection vectors
Specifications
Species E. coli
Strain HB101 Derived
Efficiency >1 x 108 cfu/ μg (HYE007-S08-10VL / FYE307-10VL / FYE307-80VL)
>1 x 109 cfu/ μg (HYE007-S08-80VL)
Format Tube (100 μL)
Quantity 10 x 100 μL、80 x 100 μL
Store at -70°C.
Content
- 100 µL / vial ECOSTM Competent Cells
- 5 µL pUC19 Control plasmid (10-4 μg / µL)
- ECOSTM patented protocol
