ECOS™ Blue Competent Cells [XL1-Blue]
Description
XL1-Blue is the most popular strain for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZΔM15 gene on the F´ episome allows blue-white color screening.
Features
- A strain modified to be ideal for blue/white screening
- Tetracycline resistant
Genotype
recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’proABlacIqZDM15 Tn10 (TetR)]
Applications
- Routine cloning application
- Subcloning
- cDNA library
- Blue/white screening
Specifications
Species E. coli
Strain XL1-Blue
Efficiency • >1 x 108 cfu/μg (FYE107-10VL / FYE107-80VL / FYE107-96WL)
• >5 x 108 cfu/μg (FYE108-10VL / FYE108-80VL / FYE108-96WL)
• >2 x 109 cfu/μg (FYE109-10VL / FYE109-80VL / FYE109-96WL)
Format Tube (100 μL) / 96-well plate (50 μL)
Quantity 10 x 100 μL、80 x 100 μL、50 μL /well
Store at -70°C.
Content
- 100 µL / vial ECOSTM Competent Cells
- 5 µL pUC19 Control plasmid (10-4 μg / µL)
- ECOSTM patented protocol
