ECOS™ 9-5 Competent Cells [JM109]
Description
JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA and the production of ssDNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.
This product can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.
Genotype
e14–(McrA–) recA1 endA1 gyrA96 thi-1 hsdR17(rK– mK+) supE44 relA1 D(lac–proAB) [F’ traD36 proAB lacIqZDM15]
Features
- Rapid growing strain
- Carries F’ episome
Applications
- Preparation of ssDNA
- Construction of gene library
- Subcloning
Specifications
Species E. coli
Strain JM109
Efficiency • > 5 x 107 cfu/μg (FYE707-10VL / FYE707-80VL / FYE707-50-20VL / FYE707-96WL)
• > 1 x 108 cfu/μg (FYE708-10VL / FYE708-80VL / FYE708-96WL)
• > 5 x 108 cfu/μg (FYE709-10VL / FYE709-80VL / FYE709-96WL)
Format Tube (100 μL) / 96-well plate (50 μL)
Quantity 10 x 100 μL、80 x 100 μL、50 μL /well
Store at -70°C.
Content
- 100 µL / vial ECOSTM Competent Cells
- 5 µL pUC19 Control plasmid (10-4 μg / µL)
- ECOSTM patented protocol
