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ECOS™ 21 Competent Cells [BL21(DE3)]
Lack of "Lon" and "Omp" proteases allows for high-level protein expression！
More Details
Description
This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.
Genotype
F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Features
- lack of Lon and Omp proteases allows high-level protein expression and accumulation
- Easy gene induction
Applications
- IPTG induced gene expression in E. coli
- Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors
- Subcloning
Specifications
Species E. coli
Strain BL21(DE3)
Efficiency > 2 x 107 cfu/μg
(FYE207-5VL / FYE207-40VL / FYE207-96WL)
Format Tube (100 μL) / 96-well plate (50 μL)
Quantity 5 x 100 μL、40 x 100 μL、50 μL /well
Store at -70°C.
Content
- 100 µL / vial ECOSTM Competent Cells
- 5 µL pUC19 Control plasmid (10-4 μg / µL)
- ECOSTM patented protocol
