ECOS™ 101 Competent Cells [DH5α]
Description
DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).
Genotype
endA1 recA1 relA1 deoR gyrA96 nupG hsdR17(rk–, mk+) supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 lacZΔM15 λ– F–
Features
Suitable for cloning with large plasmid and cDNA library construction, and also allows for blue-white colony screening.
Applications
- Cloning and subcloning
- Scale-up application
- Blue/white screening
Specifications
Species E. coli
Strain DH5α
Efficiency • >1 x 108 cfu/μg (FYE607-10VL / FYE607-80VL / FYE607-96WL)
• >5 x 108 cfu/μg (FYE678-10VL / FYE678-80VL / FYE678-96WL)
• >1 x 109 cfu/μg (FYE609-10VL / FYE609-80VL / FYE609-96WL)
• >5 x 108 cfu/μg (FYE678-10VL / FYE678-80VL)
Format Tube (100 μL) / 96-well plate (50 μL)
Quantity 10 x 100 μL、80 x 100 μL、50 μL /well
Store at -70°C.
Content
- 100 µL / vial ECOSTM Competent Cells
- 5 µL pUC19 Control plasmid (10-4 μg / µL)
- ECOSTM patented protocol
